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Promega m-mlv rt 5x reaction buffer
M Mlv Rt 5x Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5x+rt-buffer/mgcl2/pm40659902-129-3-26
Average 90 stars, based on 1 article reviews
m-mlv rt 5x reaction buffer - by Bioz Stars, 2026-10
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Article Title: Long-term azithromycin in cystic fibrosis: another possible mechanism of action?
Article Snippet: In cystic fibrosis (CF) the defective activity of the CF Transmembrane Conductance Regulator protein (CFTR) implies altered ion transport in the airways, reflecting the combined defects of a failure to secrete Cland an accelerated Na+ transport 1.. These factors ultimately lead to chronic lung infection and inflammation 2.. In diffuse panbronchiolitis (DPB), a disease which shares similarities with CF 3, macrolides improve survival, as in patients with chronic Pseudomonas aeruginosa infection 4.

Article Title: Host Genetics in Granuloma Formation: Human-Like Lung Pathology in Mice with Reciprocal Genetic Susceptibility to M. tuberculosis and M. avium
Article Snippet: Samples were chilled on ice for 3 min, and 8 μl RT mix (4 μl 5x RT-buffer, 2 μl 10 mM dNTPs, 1 μl RNasin Plus RNAase inhibitor and 1 μl M-MLV reverse transcriptase; all components from Promega) were added.

Article Title: The Centrosomal Protein Pericentrin Identified at the Basal Body Complex of the Connecting Cilium in Mouse Photoreceptors
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40°C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 μg of total RNA (whole tissue) or the complete RNA (microdissected tissue).

Article Title: Molecular analysis of the role of osmolyte transporters opuCA and betL in Listeria monocytogenes after cold and freezing stress.
Article Snippet: Listeria monocytogenes is a Gram-positive bacterium that can be found in a wide range of environments, including food, soils, plants and hard surfaces (Montville and Matthews 2008).. This bacterium is ubiquitous in the environment and is a facultative intracellular pathogen of humans (Barbuddhe and Chakraborty 2009).. It causes a rare but severe disease, with a relatively high mortality rate in populations at risk, such as old people and immunocompromised individuals.

Article Title: microRNAs in Macrobrachium olfersii embryos: Identification, their biogenesis components and potential targets.
Article Snippet: In embryonic development, microRNAs (miRNAs) regulate the complex gene expression associated with the complexity of embryogenesis.. Today, few studies have been conducted on the identification of miRNAs and components of miRNA biogenesis on embryonic development in crustaceans, especially in prawns.. In this context, the aim of this study was to identify in silico components of miRNA biogenesis, and miRNAs and potential target genes during embryonic development in the prawn Macrobrachium olfersii through small RNAs and transcriptome analyses.

Article Title: Detection of Viral −RNA and +RNA Strands in Enterovirus-Infected Cells and Tissues
Article Snippet: Reagent Amount (μl) per sample Final concentration Origin Nuclease free water 9.0 - Alfa Aesar (Ref. J71786) 5x RT-buffer 8.0 1x Promega (Ref. M5301) 2.5 mM dNTP mix with dATP, dTTP, dCTP and dGTP 8.0 0.5 mM each Promega (Ref. U1330) 10 μM ENRI (3+ or 4-) primer 4.8 1.2 μM Thermo Scientific Custom Standard DNA Oligos RNase inhibitor 0.1 4 units Promega (Ref. N2518) RT-enzyme (M-MLV RT [H–]) 0.1 20 units Promega (Ref. M530A) RNA template 10.0 - Viral RNA extraction Total 40.0 - - Supplementary table 4.

Article Title: Angiotensin-Receptor-Associated Protein Modulates Ca 2+ Signals in Photoreceptor and Mossy Fiber cells.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed for 30 min at 42 °C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and complete RNA from microdissected tissue.

Article Title: The centrosomal protein pericentrin identified at the basal body complex of the connecting cilium in mouse photoreceptors.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40uC using smaller 225 kDa Pcnt form, most likely a variant of Pcnt S, is much stronger expressed than the larger Pcnt B 360 kDa form. doi:10.1371/journal.pone.0026496.g008 PLoS ONE | www.plosone.org 11 October 2011 | Volume 6 | Issue 10 | e26496 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 mg of total RNA (whole tissue) or the complete RNA (microdissected tissue).

RNA Extraction:

Article Title: Long-term azithromycin in cystic fibrosis: another possible mechanism of action?
Article Snippet: In cystic fibrosis (CF) the defective activity of the CF Transmembrane Conductance Regulator protein (CFTR) implies altered ion transport in the airways, reflecting the combined defects of a failure to secrete Cland an accelerated Na+ transport 1.. These factors ultimately lead to chronic lung infection and inflammation 2.. In diffuse panbronchiolitis (DPB), a disease which shares similarities with CF 3, macrolides improve survival, as in patients with chronic Pseudomonas aeruginosa infection 4.

Article Title: Host Genetics in Granuloma Formation: Human-Like Lung Pathology in Mice with Reciprocal Genetic Susceptibility to M. tuberculosis and M. avium
Article Snippet: Samples were chilled on ice for 3 min, and 8 μl RT mix (4 μl 5x RT-buffer, 2 μl 10 mM dNTPs, 1 μl RNasin Plus RNAase inhibitor and 1 μl M-MLV reverse transcriptase; all components from Promega) were added.

Article Title: The Centrosomal Protein Pericentrin Identified at the Basal Body Complex of the Connecting Cilium in Mouse Photoreceptors
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40°C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 μg of total RNA (whole tissue) or the complete RNA (microdissected tissue).

Article Title: Molecular analysis of the role of osmolyte transporters opuCA and betL in Listeria monocytogenes after cold and freezing stress.
Article Snippet: Listeria monocytogenes is a Gram-positive bacterium that can be found in a wide range of environments, including food, soils, plants and hard surfaces (Montville and Matthews 2008).. This bacterium is ubiquitous in the environment and is a facultative intracellular pathogen of humans (Barbuddhe and Chakraborty 2009).. It causes a rare but severe disease, with a relatively high mortality rate in populations at risk, such as old people and immunocompromised individuals.

Article Title: microRNAs in Macrobrachium olfersii embryos: Identification, their biogenesis components and potential targets.
Article Snippet: In embryonic development, microRNAs (miRNAs) regulate the complex gene expression associated with the complexity of embryogenesis.. Today, few studies have been conducted on the identification of miRNAs and components of miRNA biogenesis on embryonic development in crustaceans, especially in prawns.. In this context, the aim of this study was to identify in silico components of miRNA biogenesis, and miRNAs and potential target genes during embryonic development in the prawn Macrobrachium olfersii through small RNAs and transcriptome analyses.

Article Title: Detection of Viral −RNA and +RNA Strands in Enterovirus-Infected Cells and Tissues
Article Snippet: Reagent Amount (μl) per sample Final concentration Origin Nuclease free water 9.0 - Alfa Aesar (Ref. J71786) 5x RT-buffer 8.0 1x Promega (Ref. M5301) 2.5 mM dNTP mix with dATP, dTTP, dCTP and dGTP 8.0 0.5 mM each Promega (Ref. U1330) 10 μM ENRI (3+ or 4-) primer 4.8 1.2 μM Thermo Scientific Custom Standard DNA Oligos RNase inhibitor 0.1 4 units Promega (Ref. N2518) RT-enzyme (M-MLV RT [H–]) 0.1 20 units Promega (Ref. M530A) RNA template 10.0 - Viral RNA extraction Total 40.0 - - Supplementary table 4.

Article Title: Angiotensin-Receptor-Associated Protein Modulates Ca 2+ Signals in Photoreceptor and Mossy Fiber cells.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed for 30 min at 42 °C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and complete RNA from microdissected tissue.

Article Title: The centrosomal protein pericentrin identified at the basal body complex of the connecting cilium in mouse photoreceptors.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40uC using smaller 225 kDa Pcnt form, most likely a variant of Pcnt S, is much stronger expressed than the larger Pcnt B 360 kDa form. doi:10.1371/journal.pone.0026496.g008 PLoS ONE | www.plosone.org 11 October 2011 | Volume 6 | Issue 10 | e26496 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 mg of total RNA (whole tissue) or the complete RNA (microdissected tissue).

cDNA Synthesis:

Article Title: Long-term azithromycin in cystic fibrosis: another possible mechanism of action?
Article Snippet: In cystic fibrosis (CF) the defective activity of the CF Transmembrane Conductance Regulator protein (CFTR) implies altered ion transport in the airways, reflecting the combined defects of a failure to secrete Cland an accelerated Na+ transport 1.. These factors ultimately lead to chronic lung infection and inflammation 2.. In diffuse panbronchiolitis (DPB), a disease which shares similarities with CF 3, macrolides improve survival, as in patients with chronic Pseudomonas aeruginosa infection 4.

Article Title: Host Genetics in Granuloma Formation: Human-Like Lung Pathology in Mice with Reciprocal Genetic Susceptibility to M. tuberculosis and M. avium
Article Snippet: Samples were chilled on ice for 3 min, and 8 μl RT mix (4 μl 5x RT-buffer, 2 μl 10 mM dNTPs, 1 μl RNasin Plus RNAase inhibitor and 1 μl M-MLV reverse transcriptase; all components from Promega) were added.

Article Title: The Centrosomal Protein Pericentrin Identified at the Basal Body Complex of the Connecting Cilium in Mouse Photoreceptors
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40°C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 μg of total RNA (whole tissue) or the complete RNA (microdissected tissue).

Article Title: Molecular analysis of the role of osmolyte transporters opuCA and betL in Listeria monocytogenes after cold and freezing stress.
Article Snippet: Listeria monocytogenes is a Gram-positive bacterium that can be found in a wide range of environments, including food, soils, plants and hard surfaces (Montville and Matthews 2008).. This bacterium is ubiquitous in the environment and is a facultative intracellular pathogen of humans (Barbuddhe and Chakraborty 2009).. It causes a rare but severe disease, with a relatively high mortality rate in populations at risk, such as old people and immunocompromised individuals.

Article Title: microRNAs in Macrobrachium olfersii embryos: Identification, their biogenesis components and potential targets.
Article Snippet: In embryonic development, microRNAs (miRNAs) regulate the complex gene expression associated with the complexity of embryogenesis.. Today, few studies have been conducted on the identification of miRNAs and components of miRNA biogenesis on embryonic development in crustaceans, especially in prawns.. In this context, the aim of this study was to identify in silico components of miRNA biogenesis, and miRNAs and potential target genes during embryonic development in the prawn Macrobrachium olfersii through small RNAs and transcriptome analyses.

Article Title: Detection of Viral −RNA and +RNA Strands in Enterovirus-Infected Cells and Tissues
Article Snippet: Reagent Amount (μl) per sample Final concentration Origin Nuclease free water 9.0 - Alfa Aesar (Ref. J71786) 5x RT-buffer 8.0 1x Promega (Ref. M5301) 2.5 mM dNTP mix with dATP, dTTP, dCTP and dGTP 8.0 0.5 mM each Promega (Ref. U1330) 10 μM ENRI (3+ or 4-) primer 4.8 1.2 μM Thermo Scientific Custom Standard DNA Oligos RNase inhibitor 0.1 4 units Promega (Ref. N2518) RT-enzyme (M-MLV RT [H–]) 0.1 20 units Promega (Ref. M530A) RNA template 10.0 - Viral RNA extraction Total 40.0 - - Supplementary table 4.

Article Title: Angiotensin-Receptor-Associated Protein Modulates Ca 2+ Signals in Photoreceptor and Mossy Fiber cells.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed for 30 min at 42 °C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and complete RNA from microdissected tissue.

Article Title: The centrosomal protein pericentrin identified at the basal body complex of the connecting cilium in mouse photoreceptors.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40uC using smaller 225 kDa Pcnt form, most likely a variant of Pcnt S, is much stronger expressed than the larger Pcnt B 360 kDa form. doi:10.1371/journal.pone.0026496.g008 PLoS ONE | www.plosone.org 11 October 2011 | Volume 6 | Issue 10 | e26496 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 mg of total RNA (whole tissue) or the complete RNA (microdissected tissue).

Reverse Transcription:

Article Title: Long-term azithromycin in cystic fibrosis: another possible mechanism of action?
Article Snippet: In cystic fibrosis (CF) the defective activity of the CF Transmembrane Conductance Regulator protein (CFTR) implies altered ion transport in the airways, reflecting the combined defects of a failure to secrete Cland an accelerated Na+ transport 1.. These factors ultimately lead to chronic lung infection and inflammation 2.. In diffuse panbronchiolitis (DPB), a disease which shares similarities with CF 3, macrolides improve survival, as in patients with chronic Pseudomonas aeruginosa infection 4.

Article Title: Host Genetics in Granuloma Formation: Human-Like Lung Pathology in Mice with Reciprocal Genetic Susceptibility to M. tuberculosis and M. avium
Article Snippet: Samples were chilled on ice for 3 min, and 8 μl RT mix (4 μl 5x RT-buffer, 2 μl 10 mM dNTPs, 1 μl RNasin Plus RNAase inhibitor and 1 μl M-MLV reverse transcriptase; all components from Promega) were added.

Article Title: The Centrosomal Protein Pericentrin Identified at the Basal Body Complex of the Connecting Cilium in Mouse Photoreceptors
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40°C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 μg of total RNA (whole tissue) or the complete RNA (microdissected tissue).

Article Title: Molecular analysis of the role of osmolyte transporters opuCA and betL in Listeria monocytogenes after cold and freezing stress.
Article Snippet: Listeria monocytogenes is a Gram-positive bacterium that can be found in a wide range of environments, including food, soils, plants and hard surfaces (Montville and Matthews 2008).. This bacterium is ubiquitous in the environment and is a facultative intracellular pathogen of humans (Barbuddhe and Chakraborty 2009).. It causes a rare but severe disease, with a relatively high mortality rate in populations at risk, such as old people and immunocompromised individuals.

Article Title: microRNAs in Macrobrachium olfersii embryos: Identification, their biogenesis components and potential targets.
Article Snippet: In embryonic development, microRNAs (miRNAs) regulate the complex gene expression associated with the complexity of embryogenesis.. Today, few studies have been conducted on the identification of miRNAs and components of miRNA biogenesis on embryonic development in crustaceans, especially in prawns.. In this context, the aim of this study was to identify in silico components of miRNA biogenesis, and miRNAs and potential target genes during embryonic development in the prawn Macrobrachium olfersii through small RNAs and transcriptome analyses.

Article Title: Detection of Viral −RNA and +RNA Strands in Enterovirus-Infected Cells and Tissues
Article Snippet: Reagent Amount (μl) per sample Final concentration Origin Nuclease free water 9.0 - Alfa Aesar (Ref. J71786) 5x RT-buffer 8.0 1x Promega (Ref. M5301) 2.5 mM dNTP mix with dATP, dTTP, dCTP and dGTP 8.0 0.5 mM each Promega (Ref. U1330) 10 μM ENRI (3+ or 4-) primer 4.8 1.2 μM Thermo Scientific Custom Standard DNA Oligos RNase inhibitor 0.1 4 units Promega (Ref. N2518) RT-enzyme (M-MLV RT [H–]) 0.1 20 units Promega (Ref. M530A) RNA template 10.0 - Viral RNA extraction Total 40.0 - - Supplementary table 4.

Article Title: Angiotensin-Receptor-Associated Protein Modulates Ca 2+ Signals in Photoreceptor and Mossy Fiber cells.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed for 30 min at 42 °C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and complete RNA from microdissected tissue.

Article Title: The centrosomal protein pericentrin identified at the basal body complex of the connecting cilium in mouse photoreceptors.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40uC using smaller 225 kDa Pcnt form, most likely a variant of Pcnt S, is much stronger expressed than the larger Pcnt B 360 kDa form. doi:10.1371/journal.pone.0026496.g008 PLoS ONE | www.plosone.org 11 October 2011 | Volume 6 | Issue 10 | e26496 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 mg of total RNA (whole tissue) or the complete RNA (microdissected tissue).

Quantitative RT-PCR:

Article Title: Long-term azithromycin in cystic fibrosis: another possible mechanism of action?
Article Snippet: In cystic fibrosis (CF) the defective activity of the CF Transmembrane Conductance Regulator protein (CFTR) implies altered ion transport in the airways, reflecting the combined defects of a failure to secrete Cland an accelerated Na+ transport 1.. These factors ultimately lead to chronic lung infection and inflammation 2.. In diffuse panbronchiolitis (DPB), a disease which shares similarities with CF 3, macrolides improve survival, as in patients with chronic Pseudomonas aeruginosa infection 4.

Article Title: Host Genetics in Granuloma Formation: Human-Like Lung Pathology in Mice with Reciprocal Genetic Susceptibility to M. tuberculosis and M. avium
Article Snippet: Samples were chilled on ice for 3 min, and 8 μl RT mix (4 μl 5x RT-buffer, 2 μl 10 mM dNTPs, 1 μl RNasin Plus RNAase inhibitor and 1 μl M-MLV reverse transcriptase; all components from Promega) were added.

Article Title: The Centrosomal Protein Pericentrin Identified at the Basal Body Complex of the Connecting Cilium in Mouse Photoreceptors
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40°C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 μg of total RNA (whole tissue) or the complete RNA (microdissected tissue).

Article Title: Molecular analysis of the role of osmolyte transporters opuCA and betL in Listeria monocytogenes after cold and freezing stress.
Article Snippet: Listeria monocytogenes is a Gram-positive bacterium that can be found in a wide range of environments, including food, soils, plants and hard surfaces (Montville and Matthews 2008).. This bacterium is ubiquitous in the environment and is a facultative intracellular pathogen of humans (Barbuddhe and Chakraborty 2009).. It causes a rare but severe disease, with a relatively high mortality rate in populations at risk, such as old people and immunocompromised individuals.

Article Title: microRNAs in Macrobrachium olfersii embryos: Identification, their biogenesis components and potential targets.
Article Snippet: In embryonic development, microRNAs (miRNAs) regulate the complex gene expression associated with the complexity of embryogenesis.. Today, few studies have been conducted on the identification of miRNAs and components of miRNA biogenesis on embryonic development in crustaceans, especially in prawns.. In this context, the aim of this study was to identify in silico components of miRNA biogenesis, and miRNAs and potential target genes during embryonic development in the prawn Macrobrachium olfersii through small RNAs and transcriptome analyses.

Article Title: Detection of Viral −RNA and +RNA Strands in Enterovirus-Infected Cells and Tissues
Article Snippet: Reagent Amount (μl) per sample Final concentration Origin Nuclease free water 9.0 - Alfa Aesar (Ref. J71786) 5x RT-buffer 8.0 1x Promega (Ref. M5301) 2.5 mM dNTP mix with dATP, dTTP, dCTP and dGTP 8.0 0.5 mM each Promega (Ref. U1330) 10 μM ENRI (3+ or 4-) primer 4.8 1.2 μM Thermo Scientific Custom Standard DNA Oligos RNase inhibitor 0.1 4 units Promega (Ref. N2518) RT-enzyme (M-MLV RT [H–]) 0.1 20 units Promega (Ref. M530A) RNA template 10.0 - Viral RNA extraction Total 40.0 - - Supplementary table 4.

Article Title: Angiotensin-Receptor-Associated Protein Modulates Ca 2+ Signals in Photoreceptor and Mossy Fiber cells.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed for 30 min at 42 °C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and complete RNA from microdissected tissue.

Article Title: The centrosomal protein pericentrin identified at the basal body complex of the connecting cilium in mouse photoreceptors.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40uC using smaller 225 kDa Pcnt form, most likely a variant of Pcnt S, is much stronger expressed than the larger Pcnt B 360 kDa form. doi:10.1371/journal.pone.0026496.g008 PLoS ONE | www.plosone.org 11 October 2011 | Volume 6 | Issue 10 | e26496 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 mg of total RNA (whole tissue) or the complete RNA (microdissected tissue).

Variant Assay:

Article Title: Long-term azithromycin in cystic fibrosis: another possible mechanism of action?
Article Snippet: In cystic fibrosis (CF) the defective activity of the CF Transmembrane Conductance Regulator protein (CFTR) implies altered ion transport in the airways, reflecting the combined defects of a failure to secrete Cland an accelerated Na+ transport 1.. These factors ultimately lead to chronic lung infection and inflammation 2.. In diffuse panbronchiolitis (DPB), a disease which shares similarities with CF 3, macrolides improve survival, as in patients with chronic Pseudomonas aeruginosa infection 4.

Article Title: Host Genetics in Granuloma Formation: Human-Like Lung Pathology in Mice with Reciprocal Genetic Susceptibility to M. tuberculosis and M. avium
Article Snippet: Samples were chilled on ice for 3 min, and 8 μl RT mix (4 μl 5x RT-buffer, 2 μl 10 mM dNTPs, 1 μl RNasin Plus RNAase inhibitor and 1 μl M-MLV reverse transcriptase; all components from Promega) were added.

Article Title: The Centrosomal Protein Pericentrin Identified at the Basal Body Complex of the Connecting Cilium in Mouse Photoreceptors
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40°C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 μg of total RNA (whole tissue) or the complete RNA (microdissected tissue).

Article Title: Molecular analysis of the role of osmolyte transporters opuCA and betL in Listeria monocytogenes after cold and freezing stress.
Article Snippet: Listeria monocytogenes is a Gram-positive bacterium that can be found in a wide range of environments, including food, soils, plants and hard surfaces (Montville and Matthews 2008).. This bacterium is ubiquitous in the environment and is a facultative intracellular pathogen of humans (Barbuddhe and Chakraborty 2009).. It causes a rare but severe disease, with a relatively high mortality rate in populations at risk, such as old people and immunocompromised individuals.

Article Title: microRNAs in Macrobrachium olfersii embryos: Identification, their biogenesis components and potential targets.
Article Snippet: In embryonic development, microRNAs (miRNAs) regulate the complex gene expression associated with the complexity of embryogenesis.. Today, few studies have been conducted on the identification of miRNAs and components of miRNA biogenesis on embryonic development in crustaceans, especially in prawns.. In this context, the aim of this study was to identify in silico components of miRNA biogenesis, and miRNAs and potential target genes during embryonic development in the prawn Macrobrachium olfersii through small RNAs and transcriptome analyses.

Article Title: Detection of Viral −RNA and +RNA Strands in Enterovirus-Infected Cells and Tissues
Article Snippet: Reagent Amount (μl) per sample Final concentration Origin Nuclease free water 9.0 - Alfa Aesar (Ref. J71786) 5x RT-buffer 8.0 1x Promega (Ref. M5301) 2.5 mM dNTP mix with dATP, dTTP, dCTP and dGTP 8.0 0.5 mM each Promega (Ref. U1330) 10 μM ENRI (3+ or 4-) primer 4.8 1.2 μM Thermo Scientific Custom Standard DNA Oligos RNase inhibitor 0.1 4 units Promega (Ref. N2518) RT-enzyme (M-MLV RT [H–]) 0.1 20 units Promega (Ref. M530A) RNA template 10.0 - Viral RNA extraction Total 40.0 - - Supplementary table 4.

Article Title: Angiotensin-Receptor-Associated Protein Modulates Ca 2+ Signals in Photoreceptor and Mossy Fiber cells.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed for 30 min at 42 °C using 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and complete RNA from microdissected tissue.

Article Title: The centrosomal protein pericentrin identified at the basal body complex of the connecting cilium in mouse photoreceptors.
Article Snippet: Poly(dT)- and random hexamer-primed cDNA synthesis (reverse transcriptase reaction) was performed 1 hour at 40uC using smaller 225 kDa Pcnt form, most likely a variant of Pcnt S, is much stronger expressed than the larger Pcnt B 360 kDa form. doi:10.1371/journal.pone.0026496.g008 PLoS ONE | www.plosone.org 11 October 2011 | Volume 6 | Issue 10 | e26496 5x RT-buffer, a mixture of dNTPs, RNAsin (all from Promega, Mannheim, Germany) and 1 mg of total RNA (whole tissue) or the complete RNA (microdissected tissue).



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